Although application of agronomic and molecular techniques for improving crop species is well documented, these have not been routinely applied to species of ecological and environmental value. vegetative clones in terms of its performance, thirty clones and seedlings of Vermilion have been planted in a replicated trial in two locations. Considerations for Large Scale Collection of Smooth Cordgrass Seed (Spartina alterniflora.L) William Skaradek, and Noel Murray USDA-NRCS Cape May Plant Materials Center and … Mode of pollination, pollen germination, and seed set in smooth cordgrass (Spartina alterniflora, Poaceae). Large amount of variability was noted for these traits and 45 plants with consistent superior rating were selected for further evaluation. Above- and belowground biomass and nutrient pools of Spartina alterniflora (smooth cordgrass) in a South American salt marsh Author: Negrin, Vanesa L., de Villalobos, Ana E., Trilla, Gabriela González, Botté, Sandra E., Marcovecchio, Jorge E. 96 0 obj <> endobj 65(6): 1794-1807. Smooth cordgrass will tolerate regular inundations with 0 to 35 parts per thousand salinity. Agron. Ribbed mussels and Spartina alterniflora production in a New England salt marsh. Spartina brasiliensis Raddi Spartina glabra Muhl. J. Intl. Usage Requirements. endstream endobj startxref endstream endobj 97 0 obj <>/Metadata 7 0 R/Pages 94 0 R/StructTreeRoot 14 0 R/Type/Catalog/ViewerPreferences 118 0 R>> endobj 98 0 obj <>/MediaBox[0 0 612 792]/Parent 94 0 R/Resources<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 99 0 obj <>stream The properties of waves passing through rigid and flexible model vegetation as well as live Spartina alterniflora and Juncus romerianus were measured. This grass can be found growing on sandy aerobic or anaerobic soils with pHs ranging from 3.7 to 7.9. Goals / Objectives Characterize Spartina alterniflora accessions at the phenotypic and molecular level. A number of crosses have been made for initiating the recurrent selection program. Fang, X., Subudhi, P.K., Venuto, B.C. Ecology. Bertness, Mark D. 1991. Late flowering accessions were associated with increased spread and tiller density but not with the other traits. Analysis of 40 selected lines evaluated in two field trials revealed that plants selected from accessions with superior seed germination rates also showed significant differences for all other traits. Plant and Animal Genome XI, San Diego, CA, Jan 11-15, 2003, Final Abstract guide, p:113. Progress 01/01/02 to 12/31/02 Outputs Activities included collecting 50 Spartina alterniflora accessions from highly impacted dying marsh sites in Louisiana. continuous and long term program with the goal of developing seed propagated synthetic smooth cordgrass varieties which maintain a broader genetic pool.ImpactsCoastal wetland loss continues to be a major environmental problem in the state of Louisiana. In case of RAPD, 176 fragments were polymorphic where as 708 fragments were polymorphic in AFLP analysis. 0 Assessment of genetic diversity in Louisiana native smooth cordgrass (Spartina alterniflora) accessions using molecular markers. A molecular genetics laboratory has been established within the LSU Agricultural Center's Department of Agronomy to conduct research involving wetland plant species. }Pc��؆�nͿ�%ٲl�J��� �����s�;�gBx&5�3���+l$@��)�L(��f�ѸAn��2+���4�-.�i@��4S |��\R{�B��ăxG$ͤpD2L*�a�KjK�����p�PG0�h:H�4�(���).��Ng ��0����c�$��;���\^hT�Si���L���b��޼��z9K��q^�2V��fo�"��&��X=��h��̢�oŻQ�Yt�2��. Subudhi, P.K., Parami, N., Ryan, A. and Harrison, S.A. 2003. Soc. Assessment of genetic diversity in Louisiana native smooth cordgrass (Spartina alterniflora) accessions using molecular markers. スパルティナ・アルテルニフロラ(Spartina alterniflora)は、イネ科の植物で、北米東部原産。 外見はヨシに似ており、高さ2mまで成長する場合もある [2]。 干潟や川の河口など汽水域で繁茂し、見た目は日本在来植物では水辺に生えるヨシすなわちアシ(葦・芦)に類似する。 Invasive Spartina alterniflora (S. alterniflora), a native riparian species in the U.S. Gulf of Mexico, has led to serious degradation to the ecosystem and biodiversity as well as economic losses since it was introduced to China in 1979. The Jaccard similarity coefficient computed from RAPD and AFLP data ranged from 22% to 89%, indicating a high level of genetic diversity. The mean percent germination was 43 % with a range of 3 to 89%. Trachynotia alterniflora (Loisel.) Trachynotia alterniflora Steud. and Harrison, S.A. 2004. Plant and Animal Genome XI, San Diego, CA, Jan 11-15, 2003, Final Abstract guide, p:113. 154 0 obj <>stream [14512] 4. Burdick, David M. 1989. Spartina alterniflora Loisel. ex Elliott Spartina laevigata Bosc ex Link Spartina merrillii A. Chev. Atlantic cordgrass. Cameron parish. Family: Poaceae Introduction Over the last 25 p:12. Spartina alterniflora, the smooth cordgrass, saltmarsh cordgrass, or salt-water cordgrass, is a perennial deciduous grass which is found in intertidal wetlands, especially estuarine salt marshes. 165(3):395-401. Several RAPD primers were used to screen the hybrid mapping population which confirmed the hybrid nature of the population making it suitable for development of a molecular map.ImpactsCostal wetland loss continues to be a major environmental problem in the state of Louisiana. h�bbd```b``� "&�H�� ��̞"Y��9 �� - smooth cordgrass SPAL Provided by USDA NRCS Golden Meadows PMC (LAPMC). An automated system was developed and used to generate regular and random Late flowering accessions were associated with increased spread and tiller density, but not with the other traits. Click on an acronym to view each weed list, or click here for a composite list of Weeds of the U.S. 2003). This plant can be weedy or invasive according to the authoritative sources noted below.This plant may be known by one or more common names in different places, and some are listed above. Fusarium palustre was associated with the dieback of Spartina alterniflora in its native North American saltmarshes. The average seed set across selected genotypes at Grand Terre Island was 51%, ranging from 7 to 83 %. A population of 100 hybrids involving two smooth cord grass lines has been generated for construction of a genetic map to locate the QTL for seed characteristics. Smooth cordgrass (Spartina alterniflora) is used extensively for shoreline protection and tidal marsh restoration on the Atlantic and Gulf coasts because of its aggressive colonizing ability and tolerance to a wide range of salinity. Spartina alterniflora: Preserving, protecting and defending coastal Louisiana. Spartina alterniflora Loiseleur-Deslongchamps, var. Conversely, negative correlations between growth traits and rust reaction were observed. Fourteen RAPD primers and 26 AFLP primer combinations generated a total of 201 and 2430 DNA fragments respectively. An ADF isolated from a monocot halophyte, Spartina alterniflora (SaADF2), imparted significantly higher level of drought and s … Actin-depolymerizing factors (ADFs) maintain the cellular actin network dynamics by regulating severing and disassembly of actin filaments in response to environmental cues. Progress made by the end of this project includes completion of the phenotypic and molecular characterization of S. alterniflora lines, selection of superior lines and polymorphism survey of the parents for construction of the Spartina molecular map. Spartina alterniflora is an invasive grass that has spread to more than 10,000 acres in Washington state and more than 1,000 acres in San Francisco Bay and tidelands. The average seed set was 49% with a range of 0 to 94%. Spartina alterniflora is a perennial grass that expands via underground rhizomes. It dominates the marshes in its native range. Spartina alterniflora has also been found to hybridize with S. foliosa, producing offspring that may be an even greater threat than S. alterniflora by itself. The mean percent germination was 43 % with a range of 3 to 89%. Compared to the original 126 native accessions, these results demonstrated a significant improvement. Ecology. [6] The hybrid can physically modify the environment to the detriment of native species, [ 7 ] and the hybrid populations have spread into creeks , bays , and more remote coastal locations. %PDF-1.7 %���� Eight hundred mutagenized plants were produced from two different mutagens; Ethyl Methane Sulphonate and Sodium Azide, and were evaluated in a marsh location in The overall objective of this project is to develop improved smooth cordgrass lines to prevent the coastal erosion as well as reclaim the eroded marsh areas. The differences observed in clustering pattern in both studies indicated that the use of molecular markers was an effective means to assess genetic diversity. Phytopathology 93:S74 (Abstr.). The differences in clustering pattern indicated the potential of molecular markers in accurate assessment of genetic diversity. Native plant Phragmites australis was also found to die back in the Dongtan wetland of the Chinese Yangtze River estuary invaded by non‐native Spartina alterniflora. Louisiana Agriculture 46 (4):42-44. Data Source and References for Spartina alterniflora (smooth cordgrass) from the USDA PLANTS database Name Search name search type enter a search name h��Xmo�6�+��b�ė�PH�5�tC�. The Jaccard similarity coefficient computed from RAPD and AFLP data ranged from 22% to 89%, indicating a high level of genetic diversity. common cordgrass Spartina anglica C.E. Additionally, fingerprinting and field evaluation of a sample of 44 clones collected from brown marsh areas are under progress. ����I0��$��[-D2j�H�@�� D�ƁH�o@��a&�ރmg`V��Cw �g� Hubbard denseflower cordgrass Spartina densiflora Brongn. The hybrids will be evaluated in the marsh condition, and the best performing ones will be identified for next round of crossing and selection. Compared Two field investigations, at Baton Rouge and Nevertheless, since all sampled individuals represent unique genotypes and all sampled marshes exhibit relatively Spartina alterniflora commonly known as smooth cord grass is a species that inhabits marsh habitat in its native range, where introduced It is known to establish itself in wave-protected mud and sand flats and grow very quickly into dense impenetrable stands. Large amount of variability was noted for these traits and 45 plants with consistent superior growth characteristics were selected for further evaluation. Hollow stems grow from 2-4 ft. (0.6-1.2 m) tall. glabra (Muhlenberg ex Elliott) Fernald, Rhodora 18: 178. Spartina alterniflora) in June and the results in October USDA is an equal opportunity provider, employer, and lender. Spartina alterniflora Loisel. AFLP analysis of another set of 38 In the southernmost part of coast of China, two height forms of Spartina alterniflora, tall and short, have invaded Leizhou Peninsula within the last decade. Root aerenchyma development in Spartina5 Plant Sci. 1916. Preliminary results show considerable natural variation among the original collections across south Louisiana for traits contributing to plant establishment from seed. Research Project: Use of Classical and Molecular Technologies for Developing Aflatoxin Resistance in Crops Location: Food and Feed Safety Research Title: An actin-depolymerizing factor from the halophyte smooth cordgrass, Spartina alterniflora (SaADF2), is superior to its rice homolog (OsADF2) in conferring drought and salt tolerance when constitutively overexpressed in rice BASIONYM: Spartina glabra Muhlenberg ex … S. alterniflorahas been introduced to new regions both intentionally and unintentionally. Development of smooth cordgrass lines with improved seed producing ability and other desirable characteristics will accelerate the on-going restoration projects by providing options for a range of improved plant materials.Publications, Progress 01/01/02 to 12/31/02OutputsCoastal erosion and wetland deterioration are serious and widespread in the state of Louisiana. 2011). Proceedings, Southern Branch Amer. Several RAPD primers were used to screen the hybrid mapping population which confirmed the hybrid nature of the population making it suitable for development of a molecular map. Rescuing the coast with biotechnology. Conduct molecular, breeding, and cultural studies with Spartina alterniflora, Schoenoplectus californicus, and Uniola paniculata; and 3. Bertness, Mark D. 1984. Spartina alterniflora is known to reproduce vegetatively via rhizomes (Callaway and Josselyn 1992; Proffitt et al. Analysis of another set of 38 S. alterniflora plants collected from brown marsh areas using AFLP technique indicated existence of unique genetic variation which will be useful in breeding superior plants with tolerance to abiotic stresses. Observations were recorded two times on vigor, spread, tillering, and rust infection on individual plants. Ryan, A.B., Subudhi, P.K., Venuto, B.C., Harrison, S.A. and Shadow, R.A. 2003. h�b```a``�"e�@����(����3 � (����� �+�� -�"`�?Y�2�a^���\�����Μq/E���J:Q�I����lL��4#��fN&{)�(S@� ~�n alterniflora using RAPD (Random Amplified Polymorphic DNA) and AFLP (Amplified Fragment Length Polymorphism) DNA markers have been standardized. This is a Spartina glabra Muhl. Spartina alterniflora Taxonomía Reino: Plantae División: Fanerógama Magnoliophyta Clase: Liliopsida Orden: Cyperales Familia: Poaceae Subfamilia: Chloridoideae Tribu: Spartina alterniflora (espartillo de cangrejal o borraza) es una gramínea perenne caducifolia que se halla en áreas inundadas intermareales, especialmente estuarios saladares. Zonation of Spartina patens and Spartina alterniflora in a New England salt marsh. The average seed set at Baton Rouge was 47% and ranged from 0 to 94%. (smooth cordgrass) (Denno 1977, 1978), with Spartina alterniflora “…an inferior host plant for development” (Denno 1977: 366). The purpose of the project is to support long-term Spartina control planning. Cluster analysis was performed on agronomic and molecular data based on genetic distance within each accession (among plants) and among accessions from the same location. Phytopathology 93:S74 (Abstr.). Bertness, Mark D. … [15194] 3. The hybrids were evaluated in the marsh, and the best performing ones were identified for next round of crossing and selection. Using RAPD and AFLP markers, 40 genotypes from seven accessions were fingerprinted. Fourteen RAPD primers and 26 AFLP primer combinations generated a total of 201 and 2430 DNA fragments respectively. released by the USDA, Natural Resources Conservation Service (NRCS), Golden Meadow Plant Materials Center in 1989. Positive correlations were found among all vegetative traits representing plant growth. Protocols for fingerprinting Spartina smooth cordgrass. Distichlis spicata (L.) Greene (saltgrass, %%EOF To download the original size of this image Using Random Amplified Fragment DNA Polymorphism (RAPD) and Amplified Fragment Length Polymorphism (AFLP) markers, 40 genotypes from seven accessions were fingerprinted. Spartina has infested Willapa Bay, Washington, and other natural settings and done great damage to the natural marine environment. The transgenic plants showed salt tolerance, and other physiological adaptations such as reduced stomatal density and closure of stomata to overcome stress. Observations were recorded two times on vigor, spread, tillering and rust infection on individual plants in the mutagenized population. Maintenance of adequate vegetation in the existing and newly restored marshlands can help slow down the coastal wetland loss. Spartina alterniflora Loisel. Significant progress has been made during the first year of the initiation of this project. Development of smooth cordgrass lines with improved seed producing ability and other desirable growth characteristics and stress tolerance will accelerate the on-going restoration projects by providing options for a range of improved plant materials, thus directly impacting the economy and environment of the state of Louisiana.Publications, Progress 01/01/03 to 12/31/03OutputsThe overall goal of this project is to develop improved smooth cordgrass lines for utilization in coastal restoration projects. Progress 09/01/01 to 03/31/04OutputsThe overall goal of this project is to apply plant breeding and genetics principles to develop improved smooth cordgrass (Spartina alterniflora) lines for utilization in coastal restoration projects. However, the effect of different height forms of Spartina alterniflora on plant–microbe interaction has not been clarified. This study validated the possibility of transforming the abandoned S. alterniflora into a biological resource to inhibit M. aeruginosa blooms through allelopathy. For a current distribution map, please consult the Plant Profile page for this species on the PLANTS Website. Grande Terre, Louisiana, showed that seed set varied greatly both within and among accessions. Mutation experiment has been initiated and 800 M1 plants from two different mutagens; Ethyl Methane Sulphonate and Sodium Azide have been evaluated in a marsh location, and in the first cycle, 50 lines have been selected based on their growth, vigor and seed set characteristics for selfing, crossing and further evaluation.ImpactsMaintenance of adequate vegetation in the existing and newly restored marshlands can help slow down the coastal wetland loss in the state of Louisiana. ‘Vermilion’ smooth cordgrass (Spartina alterniflora) is cultivar of smooth cordgrass (Spartina alternifloraLois.) A number of crosses were made each year in the recurrent selection program. The Microcystis aeruginosa (M. aeruginosa) blooms and Spartina alterniflora (S. alterniflora) invasion have caused serious damage to local ecological environment. (Poaceae, saltmeadow cordgrass), Spartina alterniflora Loisel. S. alterniflora plants collected from brown marsh areas indicated existence of unique genetic variation which will be useful in breeding superior plants to resist brown marsh phenomenon. To determine if Vermilion (a released clone commonly used in restoration projects) seedlings are comparable to Wave attenuation characteristics for rigid and flexible model vegetation as well as live Spartina alterniflora and Juncus roemerianus, two common species along coastal Mississippi and Louisiana, were investigated under both Alterniflora ) accessions using molecular markers was an effective means to assess genetic diversity reaction observed. Xi, San Diego, CA, Jan 11-15, 2003, Final guide. 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